A synonymous mutation of uncoupling protein 2 (UCP2) gene is associated with growth performance, carcass characteristics and meat quality in rabbits
نویسندگان
چکیده
BACKGROUND Uncoupling proteins 2 (UCP2) plays an important role in energy regulation, previous studies suggested that UCP2 is an excellent candidate gene for human obesity and growth-related traits in cattle and chicks. The current study was designed to detect the genetic variation of UCP2 gene, and to explore the association between polymorphism of UCP2 gene and growth, carcass and meat quality traits in rabbits. RESULTS A synonymous mutation in exon 1 and four variants in the first intron of the UCP2 gene were identified by using PCR-sequencing. The synonymous mutation c.72G>A was subsequently genotyped by MassArray system (Sequenom iPLEXassay) in 248 samples from three meat rabbit breeds (94 Ira rabbits, 83 Champagne rabbits, and 71 Tianfu black rabbits). Association analysis suggested that the individuals with AA and AG genotypes showed greater 70 d body weight (P < 0.05), 84 d body weight (P < 0.01), ADG from 28 to 84 days of age (P < 0.05), eviscerated weight (P < 0.01), semi-eviscerated weight (P < 0.01) and semi-eviscerated slaughter percentage (P < 0.05), respectively. Additionally, the individuals with AA and AG genotype had a lower pH value of longissimus muscle (P < 0.01) and hind leg muscle (P < 0.05) after slaughter 24 h. CONCLUSIONS These findings indicated that UCP2 could be a candidate gene that associated with growth performance, body composition and meat quality in rabbits, and this would contribute to advancements in meat rabbit breeding practice.
منابع مشابه
Effect of Different Levels of Mushroom Waste (Agaricus bisporus) with or without Probiotic on Growth Performance, Carcass Characteristics, and Breast Meat Quality in Broiler Chickens
The aim of this study was to investigate the effect of mushroom waste and probiotic levels on growth performance, carcass characteristics, and meat quality in broiler chickens. A 2 × 3 factorial arrangement with two levels of probiotic supplementation (0 and recommended rate) and three levels of mushroom waste inclusion (0%, 3%, and 6%) was used in a completely randomized design using male broi...
متن کاملDecreased Uncoupling Protein 2 and 3 (UCP2 and UCP3) mRNA expression by endurance exercise training with and without chronic administration of nandrolone in rat heart
Introduction: The effect of regular exercise in decreasing the incidence of heart diseases is well known. The abuse of anabolic androgenic steroids (AAS) has been associated with cardiovascular disorders. Uncoupling proteins (UCPs) transport protons across the inner mitochondrial membrane thereby proton gradient can be diminished by the action of UCPs. This process will result in the uncoupl...
متن کاملEffects of Dietary Crude Protein Level and Stocking Density on Growth Performance, Nutrient Retention, Blood Profiles, and Carcass Weight of Growing-Meat Quails
Two experiments were conducted to evaluate the effect of stocking density on growing-meat quail performance (experiment I) and the effect of dietary crude protein (CP) levels and stocking density on growth performance, nutrient retention, blood metabolites, and carcass weight of growing-meat quails under high ambient temperature (experiment II). Three hundred and eighty male growing-meat quails...
متن کاملوضعیت چند شکلی پروموتور ژن UCP2 در جمعیت سالم ایرانی
Background: Uncoupling protein-2 (UCP2), one of the mitochondrial transporter memborane protiens, is suggested as a contributor gene for obesity. A common G/A polymorphism in the UCP2 promoter region is associated with obesity and diabetes.Methods: As a cross-sectional study, 75 healthy 25-64 years volunteers were randomly selected from Tehran University of Medical Sciences population Lab. DNA ...
متن کاملEffect of Lipia Citridora leaves powder on growth performance, carcass traits, blood metabolites and meat quality of broilers
Background: Since the prohibition of antibiotic growth promoter in poultry ration by the European Union, poultry producers have been trying to replace them by other growth promoters like medicinal herbs. Lipia citridora is a medicinal herb enriched by several powerful phenolic compounds and with antioxidant and antimicrobial properties which lead to enhancing appetite and growth performance o...
متن کامل